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Please use this identifier to cite or link to this item: http://hdl.handle.net/10277/242

Authors: Fanali, Gabriella
Internal Tutor: FASANO, MAURO
Title: Allostery in a monomeric protein: the case of human serum albumin.
Abstract: Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites. Its modular domain organization provides a variety of ligand-binding sites and the flexible modular structure involves more than the immediate vicinity of the binding site(s), affecting the ligand-binding properties of the full protein. Ferric human serum heme–albumin (heme– HSA) shows a peculiar spectroscopic properties that allow to investigate structural and functional aspects of the protein. Here, a characterization of the environment of the metal binding site and of its dynamics is reported. Moreover, the effect of different endogenous and exogenous ligands on the allosteric properties of HSA and link between binding sites are investigated. As a whole, the complex mechanism modulating ligand binding to HAS represents one of the most important structure-function correlations ever reported for monomeric proteins in general.
Subject MIUR : BIO/11 BIOLOGIA MOLECOLARE
Issue Date: 2010
Doctoral course: Biologia Cellulare e Molecolare
Academic cycle: 22
Publisher: Università degli Studi dell'Insubria
Citation: Fanali, G.Allostery in a monomeric protein: the case of human serum albumin. (Doctoral Thesis, Università degli Studi dell'Insubria, 2010).

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PhD thesis fanali abstract.pdftesto abstract22,82 kBAdobe PDFNot available View/Open
PhD thesis fanali scopi.pdftesto obiettivi30,39 kBAdobe PDFNot available View/Open
PhD thesis fanali conclusioni.pdftesto conclusioni23,14 kBAdobe PDFNot available View/Open
PhD thesis fanali discussione.pdftesto discussione138,58 kBAdobe PDFNot available View/Open
PhD thesis fanali frontespizio.pdftesto frontespizio63,87 kBAdobe PDFNot available View/Open
PhD thesis fanali indice.pdftesto indice25,26 kBAdobe PDFNot available View/Open
PhD thesis fanali introduzione.pdftesto introduzione4,07 MBAdobe PDFNot available View/Open
PhD thesis fanali materiali e metodi.pdftesto materiali e metodi147,01 kBAdobe PDFNot available View/Open
PhD thesis fanali prospettive.pdftesto prospettive1,27 MBAdobe PDFNot available View/Open
PhD thesis fanali bibliografia.pdftesto bibliografia95,85 kBAdobe PDFNot available View/Open
PhD thesis fanali risultati.pdftesto risultati1,57 MBAdobe PDFNot available View/Open


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