InsubriaSPACE - Thesis PhD Repository >
Insubria Thesis Repository >
01 - Tesi di dottorato >
Please use this identifier to cite or link to this item:
|Authors: ||Solinas, Marta|
|Tutor: ||MASSI, PAOLA|
|Title: ||Meccanismi cellulari implicati nell'effetto antitumorale del cannabidiolo su cellule di glioma umano U87-MG e caratterizzazione delle sue proprietà antiangiogeniche.|
|Abstract: ||In this study we evaluated the ability of the non-psychoactive cannabinoid compound cannabidiol (CBD) to limit glioma cells invasiveness and to interfere with the most relevant intracellular pathways involved in tumor development, together with its ability to modulate angiogenic process.
We found that CBD inhibited U87-MG cells invasiveness, caused a down-regulation of ERK and Akt activation, reduced HIF-1α expression and decreased the level of several proteins specifically involved in growth, invasion and angiogenesis.
CBD also induced endothelial cell cytostasis, without inducing apoptosis, inhibited their migration, invasion and sprouting in vitro, and angiogenesis in vivo, with correspondent down-modulation of several angiogenesis-related molecules.
These results provide new insight into CBD antitumor action, showing multiple tumoral features and molecular pathways affected by CBD. Moreover, its dual effect on both glioma and endothelial cells reinforces the hypothesis of its possible use as effective anticancer agent.
Finally we evaluated the antitumor effects of other non-psychoactive cannabinoid compounds, not thoroughly investigate so far.
These compounds inhibited, with different extents, glioma cell viability, migration and invasiveness, through mechanisms nor cannabinoid or vanilloid receptors-mediated. Our results contribute to clarify the pharmacology of these molecules, although further studies are needed to understand cellular mechanisms involved in their effects.|
|Keywords: ||cannabinoidi, glioma, angiogenesi, cannabidiolo.|
|Subject MIUR : ||BIO/09 FISIOLOGIA|
|Issue Date: ||2011|
|Doctoral course: ||Neurobiologia|
|Academic cycle: ||34|
|Publisher: ||Università degli Studi dell'Insubria|
|Citation: ||Solinas, M.Meccanismi cellulari implicati nell'effetto antitumorale del cannabidiolo su cellule di glioma umano U87-MG e caratterizzazione delle sue proprietà antiangiogeniche. (Doctoral Thesis, Università degli Studi dell'Insubria, 2011).|
Files in This Item:
|Phd_thesis_solinas_completa.pdf||testo completo tesi||1,56 MB||Adobe PDF||View/Open
This item is licensed under a Creative Commons License
Items in InsubriaSPACE are protected by copyright, with all rights reserved, unless otherwise indicated.