Università degli Studi dell'Insubria Insubria Space
 

InsubriaSPACE - Thesis PhD Repository >
Insubria Thesis Repository >
01 - Tesi di dottorato >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10277/837

Authors: Zuppone, Stefania
Internal Tutor: SESSA, FAUSTO
Tutor: VAGO, RICCARDO
Title: Development of saporin-based therapeutic options for the treatment of solid tumors.
Abstract: Up to now, cancer represents one of the most challenging disease to treat, mostly due to its ability to adapt and negatively respond to current available therapies. Over the past 30 years, Ribosome Inactivating Proteins (RIPs) have attracted great interest in the scientific community for their therapeutic potential. Indeed, such toxins have been extensively used as potent and versatile therapeutic weapons due to important advantages compared to chemotherapeutics: for instance, they act independently form cell cycle, killing both quiescent and dividing cells, limiting the development of cancer resistance. However, the successful application of toxins-based therapeutics to solid tumors remains to be demonstrated. In this study, we explored the potential use of the plant-RIP saporin (SAP) for the treatment of solid tumors. In particular, we propose two different strategies, in which SAP is selectively and safely conveyed to malignant cells either in the form of recombinant protein through genetically fused targeting moieties, or throughout cell-derived extracellular vesicles as vehicles. Overall our data indicate that SAP-based recombinant proteins are promising antitumoral therapeutic options. Applied as single or combined treatment, as well as used together with traditional therapeutics, they appropriately address both intra and inter- tumor heterogeneity. In addition, the use of exosomes as SAP nanocarriers is a promising strategy to improve safety and drug delivery to tumor cells.
Keywords: Toxins, cancer, targeted therapy, recombinant proteins, exosomes
Subject MIUR : BIO/10 BIOCHIMICA
Issue Date: 2019
Language: eng
Doctoral course: Medicina sperimentale e traslazionale
Academic cycle: 31
Publisher: Università degli Studi dell'Insubria
Citation: Zuppone, S.Development of saporin-based therapeutic options for the treatment of solid tumors. (Doctoral Thesis, Università degli Studi dell'Insubria, 2019).

Files in This Item:

File Description SizeFormatVisibility
PhD_Thesis_ZupponeStefania_completa.pdftesto completo tesi19,38 MBAdobe PDFavailable from: 16/1/2021 View/Open

This item is licensed under a Creative Commons License
Creative Commons


Items in InsubriaSPACE are protected by copyright, with all rights reserved, unless otherwise indicated.


Share this record
Del.icio.us

Citeulike

Connotea

Facebook

Stumble it!

reddit


 

  ICT Support, development & maintenance are provided by the AePIC team @ CILEA. Powered on DSpace Software.  Feedback